Immunodetection techniques are based on ... it may be a good idea to run a western blot first to make sure the antibody detects the correct protein and is not creating off-target signals.

A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue ...

Both technologies have pros and cons, but which works better for chemiluminescence western blotting relies on several crucial considerations. This article explores the positives and negatives of ...