Immunodetection techniques are based on ... it may be a good idea to run a western blot first to make sure the antibody detects the correct protein and is not creating off-target signals.

Both technologies have pros and cons, but which works better for chemiluminescence western blotting relies on several crucial considerations. This article explores the positives and negatives of ...

For western-blot analysis, proteins from cultured RPE were electrophoresed on 12.5% SDS-polyacrylamide gel and then electrotransferred to a nitrocellulose membrane. We carried out immunodetection ...