Decoding Protein Glycosylation by an Integrative Mass Spectrometry-Based De Novo Sequencing Strategy
Analytical Research Center for Organic and Biological Molecules, State Key Laboratory of Drug Research, Carbohydrate-Based Drug Research Center, Shanghai Institute of Materia Medica, Chinese Academy ...
Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. A key challenge in modern chemistry research is to mimic life-like functions using ...
Protein pellets were resuspended and denatured in 20 µl 7 M Urea/2M Thiourea/100 mM TEAB, pH 7.5, reduced with 2 µl of 50 mM Tris(2-carboxyethyl) phosphine (TCEP, SCIEX), pH 8.0, at 37°C for 60 min ...
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