Nature2d
Detection of protein-protein interactions using the GST fusion protein pull-down technique
If the goal is to detect specific associated proteins: Transfer the proteins from the SDS-polyacrylamide gel to a membrane and perform immunoblotting. If the goal is to determine the sizes and ...
BMJ2d
Use of 14–3–3 and other brain-specific proteins in CSF in the diagnosis of variant Creutzfeldt-Jakob disease
METHODS The CSF from 45 patients with vCJD and 34 controls were investigated for the presence of 14–3–3 by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting with chemiluminescent ...
eLife2d
Transcriptional antitermination integrates the expression of loci of diverse phage origin in the chimeric Bartonella Gene Transfer Agent BaGTA
This manuscript describes an important finding of the transcriptional control of a chimeric gene transfer agents (GTA) cluster in Bartonella by a processive anti-termination factor (BrrG). The ...
Frontiers4d
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics
Together, recent advances in modern microscopy and improved genetically encoded calcium indicators (GECIs) have revolutionized systems neuroscience by allowing chronic simultaneous optical recording ...
Frontiers4d
Aptamer-Based Western Blot for Selective Protein Recognition
Selective protein recognition is critical in molecular biology techniques such as Western blotting and ELISA. Successful detection of the target proteins in these methods relies on the specific ...