12| Extract the phosphorylated cDNA (from Step 10) with an equal volume of phenol:chloroform (1:1).Separate the unincorporated dNTPs from the cDNA by spin-column chromatography through Sephadex G ...

Antibodies were labeled by chloramine-T–catalyzed iodination and purified by gel-filtration chromatography on a PD-10 column (Sephadex G-25 M, Pharmacia Biotech, Uppsala). Binding of Ba/F3 ...

The nanoscale supramolecular therapeutics were further passed to the Sephadex G-50 column to remove free molecular subunits ... and the expected compound was purified by silica gel chromatography, ...

This article delves into advancements in green chromatography, focusing on how innovative HPLC column design can drive ...

Researchers have produced a review of chromatography media and purification processes for handling complex and super-large ...

Method optimization can also increase the sustainability of chromatography. One aspect that has a significant impact is the column’s internal diameter, or column size in general. Making a column ...

When analyzing odorants in food or its raw materials, the formation of artifacts can significantly distort the results. In a ...

The demand for skilled laboratory professionals continues to grow, and chromatography techniques remain dominant tools in nearly every industry. Whether performing a straightforward environmental Gas ...

A high-performance liquid chromatography column is the column used in analytical chemistry to separate out components in a liquid sample. On passing through the column, analytes elute at different ...

The increasing interest in bioactive peptides (BPs) for their potential in disease control and health promotion has been accompanied by a lack of scalable processes for their purification, hindering ...

Healthy male Wistar rats (200 ± 50 g) were obtained from the Southern Laboratory Animal Facility, Prince of Songkla University, Thailand. The animals were maintained at controlled room temperature (25 ...