Fluorescence intensity measurements were used to monitor the cleavage of a ubiquitin-rhodamine substrate. All activity assays were performed in black 384-well plates in assay buffer (20 mM Tris–HCl, ...

In this study, we identify an E3 ubiquitin ligase, tripartite motif-containing protein 32 (TRIM32), as a key regulator of hepatic insulin signaling that targets the insulin receptor (INSR) for ...

(I) Western blot analysis of ubiquitin in the insoluble fraction of HEK293 cells post 2-hour OGD and subsequent 0, 6, 12 hours of reperfusion. Figure 2. Impact of sPINK1-mediated Ub phosphorylation on ...

It’s taken some time, but games that center around cowboys and indulge in the Western genre are now big hits. Looking to have some fun with 'Red Dead Online'? Here's a look at the 10 best & most ...

Western movies have a special place in cinematic history, as they capture the rugged spirit of the American frontier. From intense gun-slinging showdowns to sweeping desert landscapes full of ...