Fluorescence intensity measurements were used to monitor the cleavage of a ubiquitin-rhodamine substrate. All activity assays were performed in black 384-well plates in assay buffer (20 mM Tris–HCl, ...

In this study, we identify an E3 ubiquitin ligase, tripartite motif-containing protein 32 (TRIM32), as a key regulator of hepatic insulin signaling that targets the insulin receptor (INSR) for ...

(I) Western blot analysis of ubiquitin in the insoluble fraction of HEK293 cells post 2-hour OGD and subsequent 0, 6, 12 hours of reperfusion. Figure 2. Impact of sPINK1-mediated Ub phosphorylation on ...

Both television and cinematic content of the ever-evolving Western genre have found their place in the digital library of Netflix, which houses some of the most compelling TV shows in the genre. This ...

It’s taken some time, but games that center around cowboys and indulge in the Western genre are now big hits. Looking to have some fun with 'Red Dead Online'? Here's a look at the 10 best & most ...

What are the best Western games on PC? It’s not an easy decision to make. The Wild West was a time of lawlessness, striking gold, and perilous shootouts – no wonder it’s the subject of many ...

No one wishes for their meticulously crafted look to be spoiled by surplus oil. Consequently, oil blotting sheets can be a game-changer. Created to soak up additional skin oil, these convenient ...

Western movies have a special place in cinematic history, as they capture the rugged spirit of the American frontier. From intense gun-slinging showdowns to sweeping desert landscapes full of ...