1 were obtained from the ATCC and cultured in DMEM containing 10% FBS for a maximum of 25 passages ... at OD 600 on a spectrophotometer and serial dilutions were cultured on LB agar overnight. Colony ...

Growth curve, colony formation, soft agar assay and Xenograft model were performed to identify ... UMUC3, SW780, RT4, J82, and HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) ...

MAX Efficiency DH10Bac™ Competent (Invitrogen, 10361-012) or EMBacY cells (Thermofisher, 10361012) were transformed with 40ng plasmid DNA and turbid cultures plated onto agar containing 50μg ...

Northamptonshire have re-signed Australia spin bowler Ashton Agar for the T20 Blast group stage. The 31-year-old T20 World Cup winner featured six times for the Steelbacks in the competition in 2024.

As she announced her bid for the Liberal leadership, Chrystia Freeland found herself a victim of the incivility she helped bring about in this country.

During the peer-review process the editor and reviewers write an eLife assessment that summarises the significance of the findings reported in the article (on a scale ranging from landmark to useful) ...

SA), containing 24 h cultures of HaCat cells initially plated at 1.1 × 10 5 cells/well in 90 μL Dulbecco’s Modified Eagles Medium (DMEM) containing 10% fetal calf serum (FCS). The final peptide ...

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. High-throughput measurement of cellular traction forces at the nanoscale remains a ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...