Protein pellets were resuspended and denatured in 20 µl 7 M Urea/2M Thiourea/100 mM TEAB, pH 7.5, reduced with 2 µl of 50 mM Tris(2-carboxyethyl) phosphine (TCEP, SCIEX), pH 8.0, at 37°C for 60 min ...
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