The main application of size-exclusion chromatography is the fractionation of proteins and other water-soluble polymers, while gel permeation … See more

The technique was invented in 1955 by Grant Henry Lathe and Colin R Ruthven, working at Queen Charlotte's Hospital, London. They later … See more

In real-life situations, particles in solution do not have a fixed size, resulting in the probability that a particle that would otherwise be hampered by a pore passing right by it. Also, the … See more

Biochemical applications
In general, SEC is considered a low-resolution chromatography as it does not discern similar … See more

The advantages of this method include good separation of large molecules from the small molecules with a minimal volume of eluate, and that various solutions can be applied without … See more

SEC is used primarily for the analysis of large molecules such as proteins or polymers. SEC works by trapping smaller molecules in the pores of the adsorbent ("stationary phase"). This process is usually performed within a column, which typically consists of … See more

In simple manual columns, the eluent is collected in constant volumes, known as fractions. The more similar the particles are in size the more likely they are in the same fraction and not detected separately. More advanced columns overcome this problem by … See more